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- Title
Differential inhibition of the initiation of DNA replication in stringent and relaxed strains of Escherichia coli.
- Authors
Guzman, Elena C.; Carrillo, Francisco J.; Jimenez-Sanchez, Alfonso
- Abstract
Starvation for isoleucine inhibits chromosome, minichromosome and pBR322 DNA replication in a stringent strain of E. coli, but does not do so in a relaxed mutant. Starvation for other amino acids inhibits either chromosome and minichromosome replication in both strains. From these results we conclude that oriC and pBR322 replication are stringently regulated and that isoleucine seems not to be essential for the protein synthesis required at the initiation of oriC replication. Deprivation of isoleucine in a Rel− strain gives rise to amplification of minichromosome and pBR322 with a better yield of the latter plasmid than that following treatment with chloramphenicol.
- Publication
Genetics Research, 1988, Vol 51, Issue 3, p173
- ISSN
0016-6723
- Publication type
Article
- DOI
10.1017/S0016672300024265