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- Title
Chronic oxidative stress reduces the respiratory burst response of neutrophils from beta-thalassaemia patients.
- Authors
Amer, Johnny; Fibach, Eitan
- Abstract
Beta-thalassaemia patients are susceptible to infections by mechanisms that are not fully understood. Polymorphonuclear neutrophils (PMN) destroy microbes by producing a burst of reactive oxygen species (ROS) (respiratory burst) in response to bacterial components, as well as to phorbol-myristate-acetate (PMA). In the present study, we compared ROS generation by normal andβ-thalassaemia PMN and assessed their response to PMA. Blood cells were subjected to gelatin separation, staining with dichlorofluorescin-diacetate and flow cytometry. At basal level, the fluorescence (mean fluorescence channel) of normal and thalassaemia PMN were 12·7 ± 4·5 and 95·6 ± 19·8 respectively; it changed to 283·4 ± 72·5 and 39·5 ± 14·3, respectively, upon PMA stimulation, indicating that thalassaemia PMN have a higher basal ROS but a reduced response to PMA. When normal PMN were treated with the oxidants hydrogen peroxide and butyl-hydroxyperoxide, as well as iron and haemin, which are elevated in thalassaemia, their basal ROS increased 5–22-fold, but the PMA response was abolished. Treating thalassaemic PMN with antioxidants (N-acetyl-l-cysteine or vitamins C and E) reduced their basal ROS but enhanced their PMA response. Our findings indicate that chronically stressed PMN, e.g. in thalassaemia, have reduced capacity to elicit a respiratory burst, which may compromise their antibacterial capacity, and imply prophylactic treatment with antioxidants for recurrent infections.
- Subjects
THALASSEMIA; OXIDATIVE stress; RESPIRATION; NEUTROPHILS; HEMOGLOBINOPATHY; HEMOLYTIC anemia
- Publication
British Journal of Haematology, 2005, Vol 129, Issue 3, p435
- ISSN
0007-1048
- Publication type
Article
- DOI
10.1111/j.1365-2141.2005.05463.x