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- Title
Sampling bias created by ampicillin in isolation media for Aeromonas.
- Authors
Huddleston, Jennifer R.; Zak, John C.; Jeter, Randall M.
- Abstract
Members of the bacterial genus Aeromonas are widely isolated from aquatic environments and studied in part for their ability to act as opportunistic pathogens in a variety of animals. All aeromonads, with the exception of Aeromonas trota, are generally thought to be resistant to ampicillin, so the antibiotic is frequently added to isolation medium as a selective agent. In this study, 282 aeromonads from environmental sources were isolated on a medium without ampicillin and their resistance to ampicillin determined. Of the 104 of these isolates that were judged to be independent (nonredundant), 18 (17.3%) were susceptible to ampicillin. A chi-square analysis was performed to determine the impact of ampicillin use on enumerating Aeromonas species from environmental samples. Our results indicate that, when ampicillin is used as a selective agent, a significant portion of the aeromonad population in at least some environ ments can be omitted from isolation.
- Subjects
AEROMONAS; AQUATIC biology; PATHOGENIC microorganisms; CHI-squared test; DRUG resistance in microorganisms; AEROMONADALES
- Publication
Canadian Journal of Microbiology, 2007, Vol 53, Issue 1, p39
- ISSN
0008-4166
- Publication type
Article
- DOI
10.1139/W06-103