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- Title
Isolation and PCR amplification of a species-specific oxidoreductase-coding gene region in Listeria grayi.
- Authors
Liu, Dongyou; Lawrence, Mark L; Ainsworth, A Jerald; Austin, Frank W
- Abstract
Listeria grayi is a nonpathogenic Gram-positive bacterium that demonstrates considerable similarities to other members in the genus Listeria, including the foodborne human pathogen Listeria monocytogenes and the animal pathogen Listeria ivanovii. A rapid diagnostic test to identify and diagnose listeriosis would be valuable, especially in cases where the presence of L. grayi may complicate diagnosis. This test would be based on a unique gene present in L. grayi. In this study, after comparative screening of a recombinant L. grayi DNA library by dot blot hybridization, an L. grayi specific clone (lgr20-246) with an insert of 722 bp was isolated. By applying PCR primers derived from a distinct region of the clone not shared by other bacteria, a specific band of 420 bp was amplified from the genomic DNA of L. grayi only and not of other Listeria species or common bacteria. These results suggest that the PCR assay employing primers lgr20-246F and lgr20-246R provides an independent and precise means of distinguishing L. grayi from other Listeria species and common bacteria. Therefore, it would be another useful technique for laboratory differentiation of Listeria bacteria.
- Subjects
LISTERIA; GRAM-positive bacteria; PROKARYOTES; DNA; GENES; POLYMERASE chain reaction
- Publication
Canadian Journal of Microbiology, 2005, Vol 51, Issue 1, p95
- ISSN
0008-4166
- Publication type
Article
- DOI
10.1139/W04-108