We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Optimizing Hydrolysis Conditions of β-Lactoglobulin with Trypsin to Obtain the ACE Inhibitory Peptide.
- Authors
MEI Lin; WANG Zhi-geng; WANG Zi-long; XUE Xiu-heng
- Abstract
Three-factor quadratic orthogonal rotation design and in vitro assay was adopted to optimize the hydrolysis conditions of β-lactoglobulin with trypsin for obtaining the Angiotensin-I-converting enzyme (ACE) inhibitory peptide. The results showed that the regression equation of the impacts of the substrate concentration ( X1 ), temperature ( X2 ), enzyme, and substrate mass ratio ( X3 ) on the ACE inhibition rate was Y = 50.62 - 2.33X1-1.97X2 + 5.81 X3-3.36X2X3- 6.56X22-1.96X32. The optimum conditions were as following: when substrate concentration was at 60 g/L, the hydrolysis temperature at 30 °C, enzyme and substrate mass ratio at 5.5%, the hydrolysis time in 6 h, the hydrolysis product of ACE inhibitory activity was on the maximal inhibition rate of 53.86%.
- Subjects
HYDROLYSIS; LACTOGLOBULINS; TRYPSIN; ACE inhibitors; PEPTIDES; REGRESSION analysis; TEMPERATURE effect
- Publication
Natural Product Research & Development, 2011, Vol 23, Issue 2, p295
- ISSN
1001-6880
- Publication type
Article