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- Title
A stuttering start to plant DNA barcoding: microsatellites present a previously overlooked problem in non-coding plastid regions.
- Authors
Devey, Dion S.; Chase, Mark W.; Clarkson, James J.
- Abstract
Plastid microsatellites are simple mono/dinucleotide repeats that can cause in-vitro taq polymerase errors leading to 'stutter' peaks that reduce the clarity and reliability of electropherograms. As an efficient and automated barcoding protocol requires high-quality raw data, this paper investigates the suitability of the psbA-trnH and atpF-H regions of the plastid genome for automated barcoding purposes. Amplification of 92 individuals from species pairs and triplets was undertaken. Of these, 59% of atpF-H species samples and 36% of psbA-trnH species samples showed evidence of microsatellite-induced errors in electropherogram base calling. The downstream necessity of human intervention renders these non-coding regions sub-optimal for barcoding purposes.
- Subjects
DNA; MICROSATELLITE repeats; PLASTIDS; BAR codes; TAXONOMY
- Publication
Taxon, 2009, Vol 58, Issue 1, p7
- ISSN
0040-0262
- Publication type
Article
- DOI
10.1002/tax.581003