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- Title
Promoter methylation levels of microRNA-124 in non-neoplastic rectal mucosa as a potential biomarker for ulcerative colitis-associated colorectal cancer in pediatric-onset patients.
- Authors
Koike, Yuhki; Yin, Chengzeng; Sato, Yuki; Nagano, Yuka; Yamamoto, Akira; Kitajima, Takahito; Shimura, Tadanobu; Kawamura, Mikio; Matsushita, Kohei; Okugawa, Yoshinaga; Amano, Keishiro; Okita, Yoshiki; Ohi, Masaki; Inoue, Mikihiro; Uchida, Keiichi; Hirayama, Masahiro; Toiyama, Yuji
- Abstract
Purpose: To determine the methylation level of the miR-124 promoter in non-neoplastic rectal mucosa of patients with pediatric-onset ulcerative colitis (UC) to predict UC-associated colorectal cancer (UC-CRC). Methods: Between 2005 and 2017, non-neoplastic rectal tissue specimens were collected from 86 patients with UC, including 13 patients with UC-CRC; cancer tissues were obtained from the latter group. The methylation status of the miR-124 promoter was quantified using bisulfite pyrosequencing and compared between pediatric- and adult-onset UC patients. Results: Patients with pediatric-onset UC experienced a significantly shorter disease duration than those with adult-onset UC. The levels of miR-124 promoter methylation in non-neoplastic rectal mucosa were positively correlated with the age at the diagnosis and duration of UC. The rate of increase in miR-124 methylation was accelerated in patients with pediatric-onset UC compared to those with adult-onset UC. Furthermore, the miR-124 methylation levels in non-neoplastic rectal mucosa were significantly higher in patients with UC-CRC than in those with UC alone (P = 0.02). A receiver operating characteristic analysis revealed that miR-124 methylation in non-neoplastic tissue discriminated between patients with pediatric-onset UC with or without CRC. Conclusion: miR-124 methylation in non-neoplastic rectal mucosa may be a useful biomarker for identifying patients with pediatric-onset UC who face the highest risk of developing UC-CRC.
- Subjects
COLORECTAL cancer; METHYLATION; CANCER patients; MUCOUS membranes; RECTAL cancer; RECEIVER operating characteristic curves
- Publication
Surgery Today, 2024, Vol 54, Issue 4, p347
- ISSN
0941-1291
- Publication type
Article
- DOI
10.1007/s00595-023-02738-1