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- Title
Utility of ITS1–5.8S–ITS2 and 16S mitochondrial DNA sequences for species identification and phylogenetic inference within the Rhinonyssus coniventris species complex (Acari: Rhinonyssidae).
- Authors
Manuel de Rojas; José Úbeda; Cristina Cutillas; Ma. Mora; Concepción Ariza; Diego Guevara
- Abstract
Abstract??The complete internal transcribed spacer 1 (ITS1), 5.8S rDNA, and ITS2 region of the ribosomal DNA and a 390-bp region of the 16S rDNA gene from five taxa belonging toRhinonyssus(Rhinonyssus vanellus,Rhinonyssus tringae,Rhinonyssus neglectus,Rhinonyssus echinipesfrom Kentish plover, andRhinonyssus echinipesfrom grey plover) were sequenced to examine the level of sequence variation and the taxonomic levels to show utility in phylogeny estimation. Our data show that these molecular markers can help to discriminate between species and populations included in theRhinonyssus coniventriscomplex (R. tringae,R. neglectus,R. echinipes), which are morphologically very close and difficult to separate by classic methods. A comparative study with sequences from other rhinonyssid mites previously published was also carried out. The resulting phylogenetic tree inferred from ITS1?5.8S?ITS2 region sequences obtained in this paper, together with those from other 11 taxa of rhinonyssid, shows slight differences from the current taxonomy of the Rhinonyssidae. This study appeals for the revision of the taxonomic status of theR. coniventriscomplex, as well as for the species included within it.
- Subjects
RECOMBINANT DNA; NUCLEIC acids; BIOLOGICAL evolution; ARACHNIDA; RHINONYSSIDAE
- Publication
Parasitology Research, 2007, Vol 100, Issue 5, p1041
- ISSN
0932-0113
- Publication type
Article
- DOI
10.1007/s00436-006-0356-z