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- Title
Improved alkaloid production in Catharanthus roseus suspension cell cultures by various chemicals.
- Authors
Zhao, Jian; Zhu, Wei-Hua; Hu, Qiu; He, Xing-Wang
- Abstract
Fourteen chemicals were used to treat Catharanthus roseus suspension cell cultures to improve ajmalicine, catharanthine or serpentine biosynthesis. Ajmalicine production was increased by betaine (to 55 mg l-1), n-propyl gallate (to 27 mg l-1), succinic acid (to 31 mg l-1), malic acid (to 60 mg l-1) and tetramethyl ammonium bromide (to 64 mg l-1). Ajmalicine and catharanthine yields were about 5–6 fold higher than the control. A large portion (up to 50–85%) of total indole alkaloids was released into the medium. For maximal catharanthine production, the optimal doses of malic acid and tetramethyl ammonium bromide were 50 mg l-1 and 120 mg l-1, respectively. The mechanisms which may be responsible for these treatment effects are discussed.
- Subjects
CULTURES (Biology); CATHARANTHUS; CELL culture; ALKALOIDS; BIOCHEMICAL engineering; BIOCHEMISTRY; INDOLE alkaloids
- Publication
Biotechnology Letters, 2000, Vol 22, Issue 15, p1221
- ISSN
0141-5492
- Publication type
Article
- DOI
10.1023/A:1005696929724