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- Title
Dezvoltarea şi validarea unei metode HPLC pentru determinarea amoxicilinei trihidrat, colistinului sulfat, nipaginului şi nipasolului dintr-o suspensie injectabilă.
- Authors
Tauber, Violeta; Chiurciu, Viorica
- Abstract
A liquid chromatographic method has been developed and validated for simultaneous determination of amoxicillin trihydrate, colistin sulphate, nipasol and nipagin in injectable suspension. Efficient chromatographic separation was achived on a Hypersil Gold (150mm x 4.6mm, 5.0 μm) with mobile phase containing 4.46 g‰, pH 2.5 (adjusted with dilute sulphuric acid) in gradient with acetonitrile at a flow rate of 1.0 mL/min. detection of the analyses was performed at different wavelengths using a DAD detector. The elution was a seven step gradient elution program in 42 minutes. The proposed HPLC method was statistically validated with respect to specificity, linearity, limits of detection and quantification, ranges, precision and accuracy. The HPLC method was applied to injectable suspension in which the analyses were successfully quantified with no interfering peaks from excipients.
- Publication
Veterinary Drug / Medicamentul Veterinar, 2016, Vol 10, Issue 1, p65
- ISSN
1843-9527
- Publication type
Article