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- Title
葡萄 CYP707A 基因家族的鉴定及对果实成熟的 功能验证.
- Authors
龚丽丽; 余花; 杨杰; 陈天池; 赵双滢; 吴月燕
- Abstract
[Objective] CYP707A is a subfamily member of cytochrome P450 family, encoding ABA8'-hydroxylase, which is a key enzyme in ABA catabolism and plays an important role in plant growth and development. Identification of grape(Vitis vinifera L.)CYP707A gene family may provide theoretical basis for further study of grape fruit ripening mechanism and biological breeding techniques. [Method] Bioinformatics methods were used to analyze the gene structure, protein motif, chromosome localization, collinearity, phylogenetic relationship and cis-acting elements of promoter region of CYP707A family gene in the grape. RT-qPCR was used to analyze the spatiotemporal expression patterns of VvCYP707As gene in tissues, different grape varieties(early and medium maturity variety)and in response to the exogenous ABA treatment were analyzed by. Fruit transient transfection was applied to verify the function of Vitvi07g01751. [Result] There were five VvCYP707As genes identified in grape genome, located on five chromosomes, among which Vitvi02g01269, Vitvi07g01751 and Vitvi18g00792 were fragment replicators. The CYP707A gene family had a high degree of conservation, sharing similarities in gene structure and protein motifs, and all subcellular localization were predominantly localized to the endoplasmic reticulum. The cis-acting elements of growth, development and hormone response were significantly enriched in the promoter regions of the CYP707A gene family, containing ABA hormone-corresponding elements. Furthermore, the RT-qPCR analysis demonstrated that VvCYP707A genes demonstrated both spatiotemporal- and tissue-specific expression patterns, with elevated expression levels during the early and expansion stages of fruit development as well as in stem and leaf tissues. Notably, Vitvi07g01751 gene was highly expressed in the fruits of the early maturity variety ‘Yong Zaohong’, and the expression pattern was consistent between these two varieties. The expression pattern of Vitvi07g01751first increased and then decreased with fruit development. Under exogenous ABA treatment, VvCYP707A showed two distinct expression patterns: it induced the high expression of 5 VvCYP707A genes in the grape flesh while significantly suppressing the expressions of these genes in the grape pericarp. Furthermore, upon transient expression of Vitvi07g01751 in the grape flesh, there was a slow accumulation of sugar content, accompanied by a significant upregulation of the ABA receptor VvPYL4 gene, suggesting that Vitvi07g01751 mediated ABA regulation of grape fruit ripens. [Cconclusion] Vitvi07g01751 plays an important role in fruit ripening of grape.
- Publication
Biotechnology Bulletin, 2024, Vol 40, Issue 2, p160
- ISSN
1002-5464
- Publication type
Article
- DOI
10.13560/j.cnki.biotech.bull.1985.2023-0856