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- Title
Genetic diversity and phylogenetic analysis of Aleutian mink disease virus isolates in north-east China.
- Authors
Leng, Xue; Liu, Dongxu; Li, Jianming; Shi, Kun; Zeng, Fanli; Zong, Ying; Liu, Yi; Sun, Zhibo; Zhang, Shanshan; Liu, Yadong; Du, Rui
- Abstract
Aleutian mink disease is the most important disease in the mink-farming industry worldwide. So far, few large-scale molecular epidemiological studies of AMDV, based on the <italic>NS1</italic> and <italic>VP2</italic> genes, have been conducted in China. Here, eight new Chinese isolates of AMDV from three provinces in north-east China were analyzed to clarify the molecular epidemiology of AMDV. The seroprevalence of AMDV in north-east China was 41.8% according to counterimmuno-electrophoresis. Genetic variation analysis of the eight isolates showed significant non-synonymous substitutions in the <italic>NS1</italic> and <italic>VP2</italic> genes, especially in the <italic>NS1</italic> gene. All eight isolates included the caspase-recognition sequence NS1:285 (DQTD↓S), but not the caspase recognition sequence NS1:227 (INTD↓S). The LN1 and LN2 strains had a new 10-amino-acid deletion in-between amino acids 28-37, while the JL3 strain had a one-amino-acid deletion at position 28 in the VP2 protein, compared with the AMDV-G strain. Phylogenetic analysis based on most of <italic>NS1</italic> (1755 bp) and complete <italic>VP2</italic> showed that the AMDV genotypes did not cluster according to their pathogenicity or geographic origin. Local and imported ADMV species are all prevalent in mink-farming populations in the north-east of China. This is the first study to report the molecular epidemiology of AMDV in north-east China based on most of <italic>NS1</italic> and the complete <italic>VP2</italic>, and further provides information about polyG deletions and new variations in the amino acid sequences of NS1 and VP2 proteins. This report is a good foundation for further study of AMDV in China.
- Subjects
MANCHURIA (China); VIRUS diversity; MINK diseases; VIRUS phylogeny; VIRUS isolation; MOLECULAR epidemiology
- Publication
Archives of Virology, 2018, Vol 163, Issue 5, p1241
- ISSN
0304-8608
- Publication type
Article
- DOI
10.1007/s00705-018-3754-5