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- Title
TGF-β Regulates Hepatocellular Carcinoma Progression by Inducing Treg Cell Polarization.
- Authors
Shen, Yinan; Wei, Yongpeng; Wang, Zhouchong; Jing, Yingying; He, Haiguan; Yuan, Jianyong; Li, Rong; Zhao, Qiudong; Wei, Lixin; Yang, Tian; Lu, Junhua
- Abstract
Background/Aims: TGF-β plays a key role in the progression of various tumors. The main objective of our study was to investigate whether TGF-β is able to regulate N-nitrosodiethylamine (DEN)-induced hepatocellular carcinoma (HCC) progression in a mouse model by inducing Treg cell polarization. Methods: HCC progression, TGF-β and Foxp3 expression levels, serum TGF-β, IL10 and GP73 levels as well as percentage of Treg cells were analyzed in healthy, HCC and HCC+SM-16 mouse groups. The effect of TGF-β on Treg cell polarization in vitro was measured by flow cytometric analysis. The expression of TGF-β and IL10 was identified by IHC in HCC patients and the correlation between TGF-β and IL10 was also assessed. Results: TGF-β expression is up-regulated in a DEN-induced HCC mouse model. TGF-β can promote the differentiation of Foxp3+CD4+ T cells (Treg cells) in vitro. However, blocking the TGF-β pathway with a specific TGF-β receptor inhibitor, SM-16, reduced HCC progression and the percentage of Treg cells in liver tissue. The correlation between TGF-β and Treg cells was also confirmed in HCC patients and the expression of both TGF-β and IL-10 was shown to be associated with HCC progression. Conclusion: TGF-β is necessary for HCC progression, acting by inducing Treg cell polarization. © 2015 S. Karger AG, Basel
- Subjects
TRANSFORMING growth factors-beta; LIVER cancer; CANCER invasiveness; T cells; N-Nitrosodiethylamine; LABORATORY mice
- Publication
Cellular Physiology & Biochemistry (Karger AG), 2015, Vol 35, Issue 4, p1623
- ISSN
1015-8987
- Publication type
Article
- DOI
10.1159/000373976