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- Title
Topical administration of Juglans regia L. leaf extract accelerates diabetic wound healing.
- Authors
Nasiry, Davood; Khalatbary, Ali Reza; Ghaemi, Alireza; Ebrahimzadeh, Mohammad Ali; Hosseinzadeh, Mohammad Hossein
- Abstract
Background: Diabetic wounds are one of the most important issues in diabetic patients. It seems that Juglans regia L. leaf with antioxidant and anti-inflammatory potentials can be profitable for healing of diabetic wounds. The aim of present study was to investigate the topical administration of Juglans regia L. leaf extract in diabetic wound healing. Methods: Seventy-five diabetic male rats were randomly divided into 5 groups (n = 15), including: untreated (Control) group, Eucerin group, 2% Juglans regia L. ointment (JRL 2%) group, 5% Juglans regia L. ointment (JRL 5%) group, and Phenytoin group as a reference drug. Sampling was performed at days 7, 14, and 21 after surgery. Evaluation tests included stereology, immunohistochemistry, molecular, and biomechanical. Results: Our results showed that the wound closure rate, volumes of newly formed of epidermis and dermis, density of fibroblasts and blood vessels, collagen deposition, density of proliferation cells, expression levels of TGF-β and VEGF genes, and biomechanical characteristics were significantly higher in extract groups compared to control and eucerin groups, however, these changes were considerable in the JRL 5% group (P < 0.05). This is while that the density of neutrophils and expression levels of TNF-α and IL-1β genes in the extract groups, especially in the JRL 5% group, were significantly reduced compared to control and eucerin groups (P < 0.05). Conclusion: Topical administration of Juglans regia L. leaf extract, especially in 5% concentration, considerably accelerates diabetic wound healing.
- Subjects
DIABETES complications; WOUND healing; DRUG efficacy; STATISTICS; COLLAGEN; INTERLEUKINS; TRANSFORMING growth factors-beta; FIBROBLASTS; BLOOD vessels; ANIMAL experimentation; ONE-way analysis of variance; IMMUNOHISTOCHEMISTRY; QUANTITATIVE research; RATS; LEAVES; DESCRIPTIVE statistics; CELL proliferation; WOUNDS &; injuries; PLANT extracts; CUTANEOUS therapeutics; DATA analysis; DATA analysis software; VASCULAR endothelial growth factors
- Publication
BMC Complementary Medicine & Therapies, 2022, Vol 22, Issue 1, p1
- ISSN
2662-7671
- Publication type
Article
- DOI
10.1186/s12906-022-03735-6