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- Title
Optimization of RAPD for fingerprinting Salmonella.
- Authors
Hilton, A. C.; Banks, J. G.; Penn, C. W.
- Abstract
Random amplification of polymorphic DNA (RAPD) is proving to be a useful technique in studying the epidemiology of micro-organisms. The technique can be troublesome and time consuming to establish due to the essentially empirical approach to optimization. By standardization of certain parameters and use of a commercially available PCR buffer optimization kit, a particularly promising primer was identified and RAPD conditions for a highly discriminatory and reproducible characterization of Salmonella isolates was achieved. In addition, a technique to obtain reproducible RAPD fingerprints of Salmonella isolates without the need to purify genomic DNA is described.
- Publication
Letters in Applied Microbiology, 1997, Vol 24, Issue 4, p243
- ISSN
0266-8254
- Publication type
Article
- DOI
10.1046/j.1472-765X.1997.00385.x