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- Title
猪伪狂犬病毒引起PK-15细胞自噬的研究.
- Authors
许长萌; 王志建; 王咪; 刘倩玉; 王先炜
- Abstract
[Objectives] The aim of this research was to investigate the viral inducible-autophagy on PK-15 cell by pseudorabies virus(PRV)and effect of the autophagy on virus replication.[Methods]In this study,Western blot,transmission electron microscopy(TEM),confocal laser scanning microscopy,and transfection were performed to confirm PRV-induced autophagy. The role of autophagy on virus replication was also analyzed by autophagy inducer(rapamycin)and autophagy inhibitor(3-methyladenine,3-MA). By silencing autophagy-related gene Beclin-1, the effect of autophagy on PRV replication was further observed.[Results] Western blot assay showed that LC3-I was significantly converted into LC3-II in PK-15 cells inoculated with PRV. While the number of auto-phagosome in PK-15 cells infected with PRV were remarkably increased by TEM. In addition,the numbers of LC3-positive puncta per cell in the PRV infected cells were more than in mock cells. These results indicated that PRV infection could induce a complete autophagic flux in PK-15 cells. Furthermore,the aforementioned studies also confirmed that there were differences between autophagy induced by epidemic strains PRV ZJ01 and traditional strains PRV LA. So,the enhancement to PRV replication by autophagy was confirmed in the pharmacological alteration assay and siRNA transfection.[Conclusions]PRV infection can induce autophagy to facilitate virus replication in PK-15 cells.
- Publication
Journal of Nanjing Agricultural University / Nanjuing Nongye Daxue Xuebao, 2018, p701
- ISSN
1000-2030
- Publication type
Article
- DOI
10.7685/jnau.201801015