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- Title
Molecular identification of Ralstonia solanacearum from tomato and its biological characteristics.
- Authors
LIU Hai-long; LI Yan-yan; ZHENG Lu; LIU Hao; HUANG Jun-bin
- Abstract
[Objective]The pathogen separation and molecular identification of the infected tomato plants were conducted and the biological characteristics were analyzed to provide theoretical references for breeding the resistant varieties and controlling the tomato bacterial wilt. [Method]Ralstonia solanacearum strains were isolated using the plate dilution method, and the 16S rDNA sequence, carbohydrate utilization, pathogenicity, phylotype and sequevar of the strains were studied. [Result]Strains Tom 1-Tom 9 isolated from tomato plants were identified as Ralstonia solanacearum based on the results of colony characteristics on TTC medium, pathogenicity inoculation test and molecular identification. The result of carbohydrate utilization and pathogenicity tests showed that the 9 strains were able to oxidize three hexoses and three disaccharides, and could strongly infect tobacco, potato, tomato, pepper and eggplant. The results of phylotype multiplex PCR indicated the specific 280 and 144 bp bands were amplified from all 9 strains, respectively. [Conclusion]Nine strains of Ralstonia solanacearum isolated from tomato infected with bacterial wilt has been identified and belong to biovar III, race 1, phylotype 1 and sequcvar 17.
- Publication
Journal of Southern Agriculture, 2015, Vol 46, Issue 3, p415
- ISSN
2095-1191
- Publication type
Article
- DOI
10.3969/j:issn.2095-1191.2015.3.415