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- Title
Combining experimental evolution with next-generation sequencing: a powerful tool to study adaptation from standing genetic variation.
- Authors
Schlötterer, C; Kofler, R; Versace, E; Tobler, R; Franssen, S U
- Abstract
Evolve and resequence (E&R) is a new approach to investigate the genomic responses to selection during experimental evolution. By using whole genome sequencing of pools of individuals (Pool-Seq), this method can identify selected variants in controlled and replicable experimental settings. Reviewing the current state of the field, we show that E&R can be powerful enough to identify causative genes and possibly even single-nucleotide polymorphisms. We also discuss how the experimental design and the complexity of the trait could result in a large number of false positive candidates. We suggest experimental and analytical strategies to maximize the power of E&R to uncover the genotype-phenotype link and serve as an important research tool for a broad range of evolutionary questions.
- Subjects
HUMAN genetic variation; SINGLE nucleotide polymorphisms; GENETIC polymorphisms; PHENOTYPES; EXPERIMENTAL design
- Publication
Heredity, 2015, Vol 114, Issue 5, p431
- ISSN
0018-067X
- Publication type
Article
- DOI
10.1038/hdy.2014.86