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- Title
Diagnostic performance of high‐throughput sequencing of the T‐cell receptor beta gene for the diagnosis of cutaneous T‐cell lymphoma.
- Authors
Zimmermann, C.; Boisson, M.; Ram‐Wolff, C.; Sadoux, A.; Louveau, B.; Vignon‐Pennamen, M.‐D.; Rivet, J.; Cayuela, J.‐M.; Dobos, G.; Moins‐Teisserenc, H.; Roelens, M.; Gruber, A.; Lebbé, C.; Bagot, M.; Battistella, M.; Mourah, S.; de Masson, A.
- Abstract
Dear Editor, The early diagnosis of cutaneous T-cell lymphoma (CTCL) may be challenging and based on a combination of clinical, biological, histological and molecular criteria.1 High-throughput sequencing (HTS) of the T-cell receptor genes allows identification and quantification of each T-cell clone through the recognition of CDR3 (complementarity-determining region 3) sequences in tissue samples.2 It can be used for the sensitive monitoring of CTCL minimal residual disease.3,4 It has also been shown that tumour clone frequency (TCF, defined as the frequency of the top T-cell clone as the percentage of all T cells in a skin sample) > 25% was an independent prognostic factor of progression-free and overall survival in early-stage mycosis fungoides (MF),5 and that it correlated with the degree of skin involvement in CTCL.6 Some studies suggested that HTS was more sensitive than standard polymerase chain reaction (PCR) for the identification of a dominant T-cell clone in early MF,2,7 whereas others found a similar sensitivity.8 However, the interpretation of HTS clonality data remains subjective, as internationally validated thresholds for the definition of a dominant T-cell clone by HTS have not been published. Diagnostic performance of high-throughput sequencing of the T-cell receptor beta gene for the diagnosis of cutaneous T-cell lymphoma Both techniques identified an identical T-cell clone in all blood-skin couples of samples, except in one blood sample in which a dominant T-cell clone was identified by HTS I TRB i , identical to the skin, but no dominant T-cell clone was identified by PCR.
- Subjects
T-cell receptor genes; NUCLEOTIDE sequencing; CUTANEOUS T-cell lymphoma; SEZARY syndrome; DIAGNOSIS; PROGNOSIS; T cell receptors
- Publication
British Journal of Dermatology, 2021, Vol 185, Issue 3, p679
- ISSN
0007-0963
- Publication type
Article
- DOI
10.1111/bjd.20432