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- Title
The immunoglobulin heavy chain super enhancer controls class switch recombination in developing B cells.
- Authors
Dauba, Audrey; Näser, Emmanuelle; Andrieux, Dylan; Cogné, Michel; Denizot, Yves; Khamlichi, Ahmed Amine
- Abstract
Class switch recombination (CSR) plays an important role in adaptive immune response by enabling mature B cells to replace the initial IgM by another antibody class (IgG, IgE or IgA). CSR is preceded by transcription of the IgH constant genes and is controlled by the super-enhancer 3′ regulatory region (3′RR) in an activation-specific manner. The 3'RR is composed of four enhancers (hs3a, hs1-2, hs3b and hs4). In mature B cells, 3'RR activity correlates with transcription of its enhancers. CSR can also occur in primary developing B cells though at low frequency, but in contrast to mature B cells, the transcriptional elements that regulate the process in developing B cells are ill-known. In particular, the role of the 3'RR in the control of constant genes' transcription and CSR has not been addressed. Here, by using a mouse line devoid of the 3'RR and a culture system that highly enriches in pro-B cells, we show that the 3'RR activity is indeed required for switch transcription and CSR, though its effect varies in an isotype-specific manner and correlates with transcription of hs4 enhancer only.
- Subjects
IMMUNOGLOBULIN class switching; B cells; SUPER enhancers; IMMUNOGLOBULIN heavy chains; B cell receptors; CELL culture; IMMUNE response
- Publication
Scientific Reports, 2024, Vol 14, p1
- ISSN
2045-2322
- Publication type
Article
- DOI
10.1038/s41598-024-57576-z