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- Title
Application of Fourier-Transform Infrared (FT-IR) Spectroscopy for Determination of Total Phenolics of Freeze Dried Lemon Juices.
- Authors
Sherazi, Syed Tufail Hussain; Bhutto, Aijaz Ahmed; Mahesar, Sarfaraz Ahmed; Bhanger, Muhammad Iqbal
- Abstract
A cost effective and environmentally safe analytical method for rapid assessment of total phenolic content (TPC) in freeze dried lemon juice samples was developed using transmission Fourier-transform infrared spectroscopy (FT-IR) in conjunction with chemometric techniques. Two types of calibrations i.e. simple Beer's law and partial least square (PLS) were applied to investigate most accurate calibration model based on region from1420 to 1330 cm-1. The better analytical performance was obtained by PLS technique coefficient of determination (R²), root mean square error of calibration (RMSEC) with the value of 0.999 and 0.00864, respectively. The results of TPC in freeze dried lemon juice samples obtained by transmission FT-IR were compared with TPC observed by Folin-Ciocalteu (FC) assay and found to be comparable. Outcomes of the present study indicate that transmission FT-IR spectroscopic approach could be used as an alternative approach in place of Folin-Ciocalteu (FC) assay which is expensive and time-consuming conventional chemical methods for determination of the total phenolic content of lemon fruits.
- Subjects
LEMON juice; FOURIER transform infrared spectroscopy; FREEZE-dried foods; PHYSIOLOGICAL effects of phenols; CHEMOMETRICS
- Publication
Journal of the Chemical Society of Pakistan, 2017, Vol 39, Issue 6, p955
- ISSN
0253-5106
- Publication type
Article