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- Title
Inhibitory Effect of Tanshinones on TNF-α-induced Inflammation in Human Aortic Endothelial Cells.
- Authors
Ziru ZHANG; Mingyi MA; Longru SUN
- Abstract
[Objectives] To study the anti-inflammatory activity and mechanism of tanshinone I, cryptotanshinone and 15,16-dihydrotanshi- none I on HAECs induced by TNF-α. [Methods] Vitamin E was used as a positive control and TNF-α-induced human aortic endothelial cells (HAECs) were selected as the inflammation model cells. The mRNA levels of 1E-6, ICAM-1, VCAM-1, and NF-κB were analyzed by quantitative RT-PCR. The protein expression of NF-κB, ICAM-1, VCAM-1 and phosphorylation of ERK1/2 were determined by Western blot. The adhesion of U937 to HAECs was assessed by BCECF/AM labeling assay. [Results] TNF-α-induced over-expression of IL-6, NF-κB, ICAM-1, and VCAM-1 in HAECs were down-regulated by tanshinone I (TAN), cryptotanshinone (CPT) and 15,16-dihydrotanshinone I (DHT) both in mRNA and protein levels, respectively. Meanwhile 15,16-dihydrotanshinone I and cryptotanshinone could inhibit phosphorylation of ERK1/2 and tanshinone I inhibited U937 adhesion to HAECs significantly. [Conclusions] Tanshinones could inhibit TNF-α induced inflammatory responses on HAECs and the mechanism might be related to inhibition of phosphorylation of ERK1/2 and blocking NF-κB signa-ling pathway.
- Subjects
TUMOR necrosis factors; INFLAMMATION; ENDOTHELIAL cells; ANTI-inflammatory agents; NF-kappa B
- Publication
Medicinal Plant, 2018, Vol 9, Issue 3, p25
- ISSN
2152-3924
- Publication type
Article
- DOI
10.19600/j.cnki.issn2152-3924.2018.03.007