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- Title
Method Optimization: Analysis of Benzbromarone and Tolfenamic Acid in Citrus Tissues and Soil Using Liquid Chromatography Coupled With Triple-Quadrupole Mass Spectrometry.
- Authors
Zhang, Dan; da Silva, Danilo R.; Garrett, Timothy J.; Gonzalez, Claudio F.; Lorca, Graciela L.
- Abstract
Herein, an analytical method was developed for extraction and quantification of benzbromarone and tolfenamic acid in citrus and soil matrices using liquid-liquid extraction followed by liquid chromatography-triple quadrupole-tandem mass spectrometry analysis. The compounds were extracted using 0.1% formic acid in 6:4 ethyl acetate and n-hexane solution, and the analytes were separated using a mixture of 0.1% formic acid in ultrapure water and 0.1% formic acid in acetonitrile as mobile phase. A six-point in-matrix calibration curve was constructed providing good linearity with coefficients of determination R 2 ≥ 0.98. The limits of detection and quantification for benzbromarone and tolfenamic acid were 3.0 and 10.0 μg/kg in roots, peel, juice, and soil, and 4.0 and 12.0 μg/kg for leaves samples, respectively. The method yielded excellent recoveries between 81.3 and 101.2%, with relative standard deviation ≤9.5% in the matrices. The developed technique provides a simple and sensitive method for the determination of the chemicals and can be applied to agricultural practices.
- Subjects
MASS spectrometry; LIQUID chromatography; MASS analysis (Spectrometry); FORMIC acid; LIQUID-liquid extraction; THIN layer chromatography; HYDROPHILIC interaction liquid chromatography
- Publication
Frontiers in Plant Science, 2020, p1
- ISSN
1664-462X
- Publication type
Article
- DOI
10.3389/fpls.2020.00222