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- Title
lincRNA-RP11400K9.4 Regulates Cell Survival and Migration of Breast Cancer Cells.
- Authors
FERNÁNDEZ-ROJAS, MIGUEL A.; MELENDEZ-ZAJGLA, JORGE; MALDONADO LAGUNAS, VILMA
- Abstract
Background/Aim: Several works in the past decades pointed out the key role of long intergenic non-coding RNA (lincRNA) in breast cancer development. Here in we report for first time the importance of deregulation of lincRNA RP11- 400K9.4 in breast cancer cells which played a role in cell survival and migration. Materials and Methods: After RP11- 400K9.4 silencing by short hairpin RNAs or overexpression by GeneBlocks, real-time quantitative polymerase chain reaction (RT-PCR), microarray, migration, proliferation and viability assay were performed. Results: RP11-400K9.4 expression was mainly in the cytoplasmic fraction in 2D culture. Overexpression of RP11-400K9.4 led to a reduction of migration by MCF-7 and MDA-MB-368 cells and an increase in cellular survival after UV-C radiation. Bioinformatic analyses highlighted irradiationinduced DNA damage, DNA repair and cell-cycle pathways as the mainly affected by RP11-400K9.4. Furthermore RT-PCR assay demonstrated the overexpression of baculoviral IAP repeat containing 3 (BIRC3) a known oncogene that promotes radiotherapy resistance through the nuclear factor kappa B (NFĸB) pathway. Conclusion: RP11-400K9.4 participates in the modulation of migration and survival processes probably via the BIRC3/NFĸB pathway.
- Subjects
CANCER cell migration; CELL migration; NF-kappa B; NON-coding RNA; CELL migration inhibition; DNA repair; HAIRPIN (Genetics)
- Publication
Cancer Genomics & Proteomics (1109-6535), 2020, Vol 17, Issue 6, p769
- ISSN
1109-6535
- Publication type
Article
- DOI
10.21873/cgp.20231