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- Title
Optimization of Process Parameters for Bioactive Metabolite Production by Nocardiopsis trehalosi VSM-13 Using Response Surface Methodology and Unstructured Kinetic Modelling.
- Authors
Managamuri, Ushakiranmayi; Vijayalakshmi, Muvva; Rama Krishna Ganduri, V. S.; Babu R., Satish; Poda, Sudhakar
- Abstract
Nocardiopsis trehalosi VSM 13, an actinobacterium isolated from marine environment was tested for the optimum culture conditions in shake-flask fermentations using one-factor-at-a-time method. Response Surface Methodology (RSM) based Central composite Design was used to design the experiments, build the model and determine the optimum conditions for the desirable responses. RSM using a full factorial Box-Behnken design evaluated the optimized process conditions as 10 days of incubation time, pH - 8.0, temperature - 35°C, fructose @ 1.5% (w/v) and yeast extract @ 1% (w/v) which influenced the bioactive metabolite production by N. trehalosi VSM 13 and RSM model obtained results (R² > 0.99) revealed a satisfactory correlation between the experimental and predicted values. Unstructured kinetic model-based parameters were also evaluated using non-linear regression method to test the fitness of the selected models. Parameters of growth and substrate utilization rates have shown excellent significant R² values of 0.996 and 0.994, respectively.
- Subjects
ACTINOBACTERIA; RESPONSE surfaces (Statistics); MATHEMATICAL optimization; FERMENTATION; DYNAMICS; SUBSTRATES (Materials science)
- Publication
Current Trends in Biotechnology & Pharmacy, 2017, Vol 11, Issue 3, p223
- ISSN
0973-8916
- Publication type
Article