We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Somatic Embryogenesis in Maize and Comparison of Genetic Variability Induced by Gamma Radiation and Tissue Culture Techniques.
- Authors
Novak, F. J.; Daskalov, S.; Brunner, H.; Nesticky, M.; Afza, R.; Dolezelova, M.; Lucretti, S.; Herichova, A.; Hermelin, T.
- Abstract
Sixteen inbred lines and one hybrid of maize were tested for their capability of somatic embryogenesis and fully developed plants could he regenerated from ten inbred lines. The highest frequency of plant regeneration was expressed in the inbred line CHI 31, and when this line was crossed with a recalcitrant, non regenerating line, the F[sub1] and BC hybrids were regenerable. The results of reciprocal crosses demonstrated that dominant nuclear genes and cyto-plasmic factors are primarily responsible for the heritable determination of embryogenic callus proliferation, and in vitro regeneration at maize plants. Somaclonal and radiation induced variability was studied in maize to assess their nature and potential contribution to plant breeding. The inbred line CHI 31 possessing a high in vitro capacity of somatic embryo formation was used as experimental material. CHI 31 plants were scaled and twelve-day old zygotic embryos irradiated with [sup62]CO gamma radiation in situ. Mature caryposes were harvested and assigned as M[sub1] material. In another series, immature zygotic embryos (size 1.2-.5 mm) were cultured in vitro on N-6 medium supplemented with 2,4-D (2.5 μM), and somatic embryos regenerated into plants these were transplanted into soil and self-pollinated. Regenerants from non -irradiated cultures were grown as R[sub1] generation, while regenerants from irradiated explants were considered as M[sub1] R[sub1] generation. The genetic variability was evaluated in the M[sub2], R[sub2] and M[sub2]R[sub2] generations, respectively, and compared with a non-treated seed control. Irradiation induced a variety of chlorophyll and morphological variants segregating in the M[sub2] generation; however, the frequency of devaint types obtained in the R[sub2] generation (somaclonal variation) was significantly exceeding the one derived from the M[sub2] populations.
- Subjects
CORN; SOMATIC embryogenesis; PLANT genetic engineering; GAMMA rays; PLANT tissue culture; PLANT physiology &; genetics
- Publication
Plant Breeding, 1988, Vol 101, Issue 1, p66
- ISSN
0179-9541
- Publication type
Article
- DOI
10.1111/j.1439-0523.1988.tb00268.x