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- Title
A urinary proteomic study in hypercalciuric dogs with and without calcium oxalate urolithiasis.
- Authors
Sumonwan Chamsuwan; Chollada Buranakarl; Angkanaporn, Kris; Thasinas Dissayabutra; Natthaya Chuaypen; Trairak Pisitkun; Nuttiya Kalpongnukul
- Abstract
Background and Aims: Hypercalciuria is an important predisposing factor commonly found in humans and dogs with calcium oxalate (CaOx) urolithiasis. Calcium oxalate crystals can induce an inflammatory reaction that subsequently produces several proteins that have an inhibitory or stimulatory effect on stone formation. This study aimed to evaluate the differences in urinary proteomic profiles between hypercalciuric CaOx stone dogs and hypercalciuric stone-free dogs (CaOx stone and control groups, respectively). Materials and Methods: Seven dogs with hypercalciuric CaOx urolithiasis and breed-, sex-, and aged-matched controls with hypercalciuria were included in the study. Serum and urine samples were obtained from all dogs to analyze electrolytes. Urinary proteomic profiles were analyzed using liquid chromatography-mass spectrometry. Student's t-test was used to compare the differences between groups. Results: Forty-nine urinary proteins were identified in the stone-free and CaOx stone groups, whereas 19 and 6 proteins were unique in the CaOx stone and stone-free groups, respectively. The urinary thrombomodulin level was significantly higher in the CaOx stone group (relative ratio = 1.8, p < 0.01) than in the stone-free group. Conclusion: This study demonstrated that urinary proteomic profiles may be used as a candidate biomarker for urinary tract injury in CaOx urolithiasis in dogs.
- Subjects
CALCIUM oxalate; DOG walking; URINARY calculi; LIQUID chromatography-mass spectrometry; PROTEOMICS; DOGS; URINARY organs
- Publication
Veterinary World, 2022, Vol 15, Issue 12, p2937
- ISSN
0972-8988
- Publication type
Article
- DOI
10.14202/vetworld.2022.2937-2944