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- Title
Effect of high-fat diet and growth stage on the diversity and composition of intestinal microbiota in healthy bovine livestock.
- Authors
Jiao, Shengyin; Cao, Hui; Dai, Yue; Wu, Junhui; Lv, Jia; Du, Renjia; Han, Bei
- Abstract
BACKGROUND This study aimed to investigate the composition of bacteria in the bovine rectum and their functions during growth, in relation to different diets. Fecal samples were collected from 6-, 12-, 18- and 24-month cattle fed high-fat diet, and healthy female parents fed regular diet. Total DNA was amplified ( V3-V4 of 16S rRNA) and submitted to barcode- DNA pyrosequencing. Intestinal microbiota profiles and functions were then analyzed. RESULTS A total of 114 512 operational taxonomic units were detected from the 1 802 243 sequences obtained. In 6-month-old and female parent groups, the top three abundant phyla were Bacteroidetes (37.6%, 32.2%), Firmicutes (34.4%, 48.2%) and Proteobacteria (9.1%, 6.3%); in the 12-, 18- and 24-month groups, they were Proteobacteria (45.5%, 47.1%, 38.8%), Firmicutes (27.4%, 22.2%, 20.1%) and Bacteroidetes (14.9%, 19.4%, 17.7%), respectively. Paludibacter and Desulfopila in abundance showed negative ( P < 0.001) and positive ( P < 0.05) correlation, respectively, to cattle weight gain through metagenomic functional prediction of methane, cysteine and methionine metabolism. Meanwhile, cofactor/vitamin and amino acid metabolic processes were significantly higher in bacteria from the regular diet group than high-fat diet groups, with markedly lower cellular processes and signaling, and reduced glycan biosynthesis and metabolism ( P < 0.01). CONCLUSIONS The 6-month cattle and female parents shared similar intestinal bacteria; the community structure of fecal microbiota was significantly affected by high-fat diet in older cattle. © 2017 Society of Chemical Industry
- Subjects
HIGH-fat diet; LIVESTOCK; LIVESTOCK diversification; METAGENOMICS; RIBOSOMAL RNA; DNA; PYROSEQUENCING; BACTEROIDETES
- Publication
Journal of the Science of Food & Agriculture, 2017, Vol 97, Issue 14, p5004
- ISSN
0022-5142
- Publication type
Article
- DOI
10.1002/jsfa.8380