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- Title
Polyadenylated tail length variation pattern in ultra-rapid vitrified bovine oocytes.
- Authors
Dutta, D. J.; Raj, Himangshu; Dev, Hiramoni
- Abstract
Aim: The current study aims at investigating the polyadenylated (poly[A]) tail length of morphologically high and low competent oocytes at different developmental stages. Furthermore, effect of ultra-rapid vitrification on the poly(A) tail length was studied. Materials and Methods: Fresh bovine cumulus oocyte complexes from abattoir originated ovaries were graded based on morphological characters and matured in vitro. Cryopreservation was done by ultra-rapid vitrification method. mRNA was isolated from different categories of oocyte and subjected to ligation-mediated poly(A) test followed by polymerase chain reaction for determining the poly(A) tail length of β actin, gap junction protein alpha 1 (GJA1), poly(A) polymerase alpha (PAPOLA), and heat shock 70 kDa protein (HSP70) transcripts. Results: GJA1, PAPOLA, and HSP70 showed significantly higher poly(A) in immature oocytes of higher competence irrespective of vitrification effects as compared to mature oocytes of higher competence. Conclusion: mRNA poly(A) tail size increases in developmentally high competent immature bovine oocytes. There was limited effect of ultra-rapid vitrification of bovine oocytes on poly(A).
- Subjects
POLYADENYLIC acid; OVUM; RNA polymerases; MESSENGER RNA; GAP junctions (Cell biology)
- Publication
Veterinary World, 2016, Vol 9, Issue 10, p1070
- ISSN
0972-8988
- Publication type
Article
- DOI
10.14202/vetworld.2016.1070-1074