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- Title
Inter-MAR Association Contributes to Transcriptionally Active Looping Events in Human β-globin Gene Cluster.
- Authors
Li Wang; Li-Jun Di; Xiang Lv; Wei Zheng; Zheng Xue; Zhi-Chen Guo; De-Pei Liu; Chi-Chuan Liang
- Abstract
Matrix attachment regions (MARs) are important in chromatin organization and gene regulation. Although it is known that there are a number of MAR elements in the β-globin gene cluster, it is unclear that how these MAR elements are involved in regulating β-globin genes expression. Here, we report the identification of a new MAR element at the LCR(locus control region) of human β-globin gene cluster and the detection of the inter-MAR association within the β-globin gene cluster. Also, we demonstrate that SATB1, a protein factor that has been implicated in the formation of network like higher order chromatin structures at some gene loci, takes part in β-globin specific inter-MAR association through binding the specific MARs. Knocking down of SATB1 obviously reduces the binding of SATB1 to the MARs and diminishes the frequency of the inter-MAR association. As a result, the ACH establishment and the a-like globin genes and β-like globin genes expressions are affected either. In summary, our results suggest that SATB1 is a regulatory factor of hemoglobin genes, especially the early differentiation genes at least through affecting the higher order chromatin structure.
- Subjects
MATRIX attachment regions; GENES; CHROMATIN; LOCUS (Genetics); GENE expression; PROTEINS
- Publication
PLoS ONE, 2009, Vol 4, Issue 2, p1
- ISSN
1932-6203
- Publication type
Article
- DOI
10.1371/journal.pone.0004629