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- Title
Phosphatidylserine Targets Single-Walled Carbon Nanotubes to Professional Phagocytes In Vitro and In Vivo.
- Authors
Konduru, Nagarjun V.; Tyurina, Yulia Y.; Weihong Feng; Basova, Liana V.; Belikova, Natalia A.; Bayir, Hülya; Katherine Clark; Marc Rubin; Donna Stolz; Helen Vallhov; Annika Scheynius; Erika Witasp; Bengt Fadeel; Padmakar D. Kichambare; Alexander Star; Elena R. Kisin; Murray, Ashley R.; Shvedova, Anna A.; Valerian E. Kagan
- Abstract
Broad applications of single-walled carbon nanotubes (SWCNT) dictate the necessity to better understand their health effects. Poor recognition of non-functionalized SWCNT by phagocytes is prohibitive towards controlling their biological action. We report that SWCNT coating with a phospholipid ''eat-me'' signal, phosphatidylserine (PS), makes them recognizable in vitro by different phagocytic cells - murine RAW264.7 macrophages, primary monocyte-derived human macrophages, dendritic cells, and rat brain microglia. Macrophage uptake of PS-coated nanotubes was suppressed by the PS-binding protein, Annexin V, and endocytosis inhibitors, and changed the pattern of pro- and anti-inflammatory cytokine secretion. Loading of PS-coated SWCNT with pro-apoptotic cargo (cytochrome c) allowed for the targeted killing of RAW264.7 macrophages. In vivo aspiration of PS-coated SWCNT stimulated their uptake by lung alveolar macrophages in mice. Thus, PS-coating can be utilized for targeted delivery of SWCNT with specified cargoes into professional phagocytes, hence for therapeutic regulation of specific populations of immune-competent cells.
- Subjects
PHOSPHATIDYLSERINES; CARBON nanotubes; PHAGOCYTES; MACROPHAGES; DENDRITIC cells; MICROGLIA; CARRIER proteins
- Publication
PLoS ONE, 2009, Vol 4, Issue 2, p1
- ISSN
1932-6203
- Publication type
Article
- DOI
10.1371/journal.pone.0004398