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- Title
Molecular Characterization and Phylogenetic Analysis of Novel α-gliadin Genes from Triticum dicoccoides L.
- Authors
Zhang, D. L.; Gao, A. L.; Li, Y. G.; Su, Y. R.; He, S. B.; Li, S. P.
- Abstract
The quality traits of Triticum dicoccoides KU-13441 (Triticum turgidum L. var. dicoccoides (AABB, 2n= 4x= 28)) were analyzed by mixograph, and the results showed that T. dicoccoides KU-13441 had desirable qualities in gluten strength and flour stirring tolerance. Subsequently, seventeen novel full-ORF αgliadin genes and thirty-five pseudogenes were cloned and sequenced from T. dicoccoides KU-13441. Among the 17 novel full-ORF αgliadin genes, the putative proteins of Gli2-TD-44 and Gli2-TD-46 contained an extra cysteine residue, located in the first nonrepetitive region and Nterminal repetitive domain, respectively, rather than in the second nonrepetitive region like other αgliadins. Prokaryotic expression analysis and western-blotting indicated that these two αgliadin genes could be successfully expressed under the control of T7 promoter. According to the varying numbers of 4 typical coeliac disease toxic peptides and glutamine residues in the two ployglutamine domains among the 17 αgliadins, Gli2- TD-39, Gli2-TD-46 and Gli2-TD-47 genes were assigned to sub-genome B and other 14 genes were assigned to sub-genome A. Phylogenetic analysis including two S-genome species, Aegilops longissima (Sl) and Aegilops speltoides (S) revealed that the αgliadin sequences of the B genome in T. dicoccoides had closer genetic relationship with those from Ae. speltoides. This implies that Ae. speltoides might participate in the origin of wheat B genome.
- Subjects
EMMER wheat; MOLECULAR phylogeny; GLIADINS; WHEAT quality; WHEAT genetics; WESTERN immunoblotting; GENE expression in plants
- Publication
Journal of Agricultural Science & Technology, 2014, Vol 16, Issue 1, p203
- ISSN
1680-7073
- Publication type
Article