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- Title
Efficient Serum-Free Derivation of Oligodendrocyte Precursors from Neural Stem Cell-Enriched Cultures.
- Authors
Rao, Rajesh C.; Boyd, Justin; Padmanabhan, Raji; Chenoweth, Josh G.; McKay, Ronald D.
- Abstract
Oligodendrocytes derived in the laboratory from stem cells have been proposed as a treatment for acute and chronic injury to the central nervous system. Platelet-derived growth factor (PDGF) receptor α (PDGFRα) signaling is known to regulate oligodendrocyte precursor cell numbers both during development and adulthood. Here, we analyze the effects of PDGFRα signaling on central nervous system (CNS) stem cell-enriched cultures. We find that AC133 selection for CNS progenitors acutely isolated from the fetal cortex enriches for PDGF-AA-responsive cells. PDGF-AA treatment of fibroblast growth factor 2-expanded CNS stem cell-enriched cultures increases nestin[sup+] cell number, viability, proliferation, and glycolytic rate. We show that a brief exposure to PDGF-AA rapidly and efficiently permits the derivation of O4[sup+] oligodendrocyte-lineage cells from CNS stem cell-enriched cultures. The derivation of oligodendrocyte-lineage cells demonstrated here may support the effective use of stem cells in understanding fate choice mechanisms and the development of new therapies targeting this cell type.
- Subjects
CENTRAL nervous system; PLATELET-derived growth factor; NEURAL stem cells; FIBROBLAST growth factors; HUMAN cell culture
- Publication
Stem Cells, 2009, Vol 27, Issue 1, p116
- ISSN
1066-5099
- Publication type
Article
- DOI
10.1634/stemcells.2007-0205