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- Title
Detection of Muramidase (Lysozyme)-secreting Leucocytes at the Single-Cell Level by a Protein A Plaque Assay.
- Authors
Smith, C. I. E.; Hammarström, L.
- Abstract
Using a modification of the protein A plaque assay, muramidase (lysozyme)-producing leucocytes were detected as plaque-forming cells. In the presence of anti-muramidase Ig and complement the secreted lysozyme resulted in lysis of protein-A-coated target erythrocytes. By the use of a monolayer technique individual plaque-forming cells could he identified by staining procedures. Granulocytes as well as monocytes were found to produce muramidase und thus lo form plaques. This method could serve as a useful tool when studying lysozyme secretion. Furthermore by the use of appropriate antisera, this method could be employed for the study of any cell type (any secretion), provided enough molecules are being secreted.
- Subjects
LEUCOCYTES; KILLER cells; ERYTHROCYTES; MONOCYTES; IMMUNE serums; BIOLOGICAL transport
- Publication
Scandinavian Journal of Immunology, 1980, Vol 11, Issue 1, p79
- ISSN
0300-9475
- Publication type
Article
- DOI
10.1111/j.1365-3083.1980.tb00211.x