We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Polyclonal Activation of Human B Lymphocytes in Vitro by Pokeweed Mitogen: a Simple Technique for the Simultaneous Assessment of Cell Proliferation, Generation of Plasma Cells, Plaque--forming Cells and Immunoglobulin Production.
- Authors
Nespoli, L.; Vitiello, A.; Maccarlo, R.; Lanzavecch, A.; Ugazio, A.G.
- Abstract
A simple technique has been worked out for the simultaneous assessment of cell proliferation, generation of plasma cells, plaque-forming cells and immunoglobulin production from single cultures of 1 × 106 human peripheral blood lymphocytes stimulated with pokeweed mitogen (PWM). Kinetic studies showed that number of cells, thymidine incorporation rate, number of plaque-forming cells against fluorescein-isothiocyanate-haptenated sheep erythrocytes and concentration of IgM and IgG in the supernatant peaked on the average between day 5 and 7 of culture. This technique is particularly suitable for the analysis of hypogammaglobulinaemias in infancy.
- Subjects
CELL proliferation; B cells; MITOGENS; PLASMA cells; IMMUNOGLOBULINS
- Publication
Scandinavian Journal of Immunology, 1978, Vol 8, Issue 6, p489
- ISSN
0300-9475
- Publication type
Article
- DOI
10.1111/j.1365-3083.1978.tb00548.x