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- Title
Human Secretory Component II. Physicochemical Characterization of Free Secretory Component Purified from Colostrum.
- Authors
Brandtzaeg, P.
- Abstract
The gel filtration behavior of purified secretory component indicated an Einstein-Stokes radius of 44 to 46 Å, a diffusion coefficient of 4.7 to 4.9 × 10-7 cm² · sec-1, and a molecular weight of about 110,000 daltons. Ultracentrifugation indicated a sedimentation coefficient of 4.8 to 5.4S and a molecular weight of about 83,000 daltons. The latter estimate was based on a partial specific volume of 0.712 ml/g, calculated from the amino acid and carbohydrate composition. Isoelectric focusing demonstrated at least six fractions with pi ranging from pH 4.75 to 5.25. Different fractions were, apparently, antigenically similar and complexed spontaneously with dimeric IgA and 19S IgM. This complexing was of slightly higher efficiency for secretory component enriched in the more basic material.
- Subjects
ELECTROPHORESIS; SOLID solutions; AMINO acids; HYDROGEN-ion concentration; ORGANIC compounds; POROUS materials
- Publication
Scandinavian Journal of Immunology, 1974, Vol 3, Issue 6, p707
- ISSN
0300-9475
- Publication type
Article
- DOI
10.1111/j.1365-3083.1974.tb01306.x