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- Title
Quantitative studies of ribosome conformational dynamics.
- Abstract
AbstractThe ribosome is a dynamic machine that undergoes many conformational rearrangements during the initiation of protein synthesis. Significant differences exist between the process of protein synthesis initiation in eubacteria and eukaryotes. In particular, the initiation of eukaryotic protein synthesis requires roughly an order of magnitude more initiation factors to promote efficient mRNA recruitment and ribosomal recognition of the start codon than are needed for eubacterial initiation. The mechanisms by which these initiation factors promote ribosome conformational changes during stages of initiation have been studied using cross-linking, footprinting, site-directed probing, cryo-electron microscopy, X-ray crystallography, fluorescence spectroscopy and single-molecule techniques. Here, we review how the results of these different approaches have begun to converge to yield a detailed molecular understanding of the dynamic motions that the eukaryotic ribosome cycles through during the initiation of protein synthesis.
- Subjects
RIBOSOMES; ORGANELLES; NUCLEOPROTEINS; PROTEIN synthesis; EUBACTERIALES; FLUORESCENCE spectroscopy
- Publication
Quarterly Reviews of Biophysics, 2007, Vol 40, Issue 2, p163
- ISSN
0033-5835
- Publication type
Article
- DOI
10.1017/S0033583507004647