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- Title
PU.1 subcellular localization in acute myeloid leukaemia with mutated NPM1.
- Authors
Pianigiani, Giulia; Betti, Camilla; Bigerna, Barbara; Rossi, Riccardo; Brunetti, Lorenzo
- Abstract
I NPM1 i is one of the most frequently mutated genes in acute myeloid leukaemia (AML), with one third of AML patients carrying I NPM1 i mutations (Falini I et al. i , [4]). We studied 17 I NPM1 i wild-type and 22 I NPM1 i -mutated samples using a commercially available PU.1 antibody (referred to as ab1), together with an antibody directed against the N-terminus of NPM1 (recognizing both wild-type and mutant forms, referred to as tNPM1). In summary, IHC of primary bone marrow biopsies did not reveal clear differences in PU.1 localization between I NPM1 i wild-type and I NPM1 i -mutated samples and was not able to demonstrate cytoplasmic localization of PU.1 in mutated cases.
- Subjects
LEUKEMIA; ORGANELLE formation; ACUTE myeloid leukemia; CELL fractionation
- Publication
British Journal of Haematology, 2020, Vol 188, Issue 1, p184
- ISSN
0007-1048
- Publication type
Article
- DOI
10.1111/bjh.16344