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- Title
Facile Determination of Sodium Ion and Osmolarity in Artificial Tears by Sequential DNAzymes.
- Authors
Eun Hye Kim; Eun-Song Lee; Dong Yun Lee; Young-Pil Kim
- Abstract
Despite high relevance of tear osmolarity and eye abnormality, numerous methods for detecting tear osmolarity rely upon expensive osmometers. We report a reliable method for simply determining sodium ion-based osmolarity in artificial tears using sequential DNAzymes. When sodium ion-specific DNAzyme and peroxidase-like DNAzyme were used as a sensing and detecting probe, respectively, the concentration of Na+in artificial tears could be measured by absorbance or fluorescence intensity, which was highly correlated with osmolarity over the diagnostic range (R2 > 0.98). Our approach is useful for studying eye diseases in relation to osmolarity.
- Subjects
DEOXYRIBOZYMES; EYE abnormalities; OSMOLAR concentration; OSMOMETERS; EYE diseases
- Publication
Sensors (14248220), 2017, Vol 17, Issue 12, p2840
- ISSN
1424-8220
- Publication type
Article
- DOI
10.3390/s17122840