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- Title
The Toxicity of a Lipid Transfer Protein ( Cc-LTP1) from Coffea canephora Seeds on the Larval Development of Callosobruchus maculatus (Coleoptera: Bruchidae).
- Authors
Zottich, Umberto; Cunha, Maura; Dias, Germana; Rabelo, Guilherme; Oliveira, Antonia; Carvalho, André; Fernandes, Kátia; Nascimento, Viviane; Gomes, Valdirene
- Abstract
In this work, we analyzed the effects of coffee seed proteins, especially Cc-LTP on the larval development of Callosobruchus maculatus (F.) (Coleoptera: Bruchidae), a bruchid pest of beans and the most important insect pest of Vigna unguiculata (L.) Walp. Artificial seed assay, which incorporated the F/0-90 fraction from Coffea canephora seeds, resulted in the reduction of oviposition and caused an inhibition of C. maculatus larval development in a dose-dependent manner. The F/0-90 fraction used at a 4 % concentration resulted in the survival of no larvae. The purified Cc-LTP, at a concentration of 0.5 %, also demonstrated effective inhibition of larval development, reducing both females oviposition and the weight and number of larvae. Cc-LTP was also able to inhibit the C. maculatus gut α-amylase activity, and immunolabeling by an anti-LTP serum was observed in the midgut tissues of the C. maculatus larvae. Cc-LTP has shown binding affinity towards microvillar cells, endoplasmic reticulum and mitochondria, as demonstrated by micrographic images taken by a transmission electron microscope. The results from this study indicate that Cc-LTP has insecticidal actions toward C. maculatus and exerts anti-nutritional effects with direct actions on intestinal tissues.
- Subjects
TOXICOLOGY; LIPID transfer protein; COFFEE; DEVELOPMENTAL biology; COWPEA weevil; INSECTICIDES
- Publication
Protein Journal, 2014, Vol 33, Issue 5, p422
- ISSN
1572-3887
- Publication type
Article
- DOI
10.1007/s10930-014-9575-9