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- Title
A biophysical miRNA-mRNA interaction model infers canonical and noncanonical targets.
- Authors
Khorshid, Mohsen; Hausser, Jean; Zavolan, Mihaela; van Nimwegen, Erik
- Abstract
We introduce a biophysical model of miRNA-target interaction and infer its parameters from Argonaute 2 cross-linking and immunoprecipitation data. We show that a substantial fraction of human miRNA target sites are noncanonical and that predicted target-site affinity correlates well with the extent of target destabilization. Our model provides a rigorous biophysical approach to miRNA target identification beyond ad hoc miRNA seed-based methods.
- Subjects
IMMUNOPRECIPITATION; PHYSICAL biochemistry; MICRORNA; ARGONAUTE proteins; RNA interference
- Publication
Nature Methods, 2013, Vol 10, Issue 3, p253
- ISSN
1548-7091
- Publication type
Article
- DOI
10.1038/nmeth.2341