We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Tomographic phase microscopy.
- Authors
Wonshik Choi; Fang-Yen, Christopher; Badizadegan, Kamran; Seungeun Oh; Niyom Lue; Dasari, Ramachandra R.; Feld, Michael S.
- Abstract
We report a technique for quantitative three-dimensional (3D) mapping of refractive index in live cells and tissues using a phase-shifting laser interferometric microscope with variable illumination angle. We demonstrate tomographic imaging of cells and multicellular organisms, and time-dependent changes in cell structure. Our results will permit quantitative characterization of specimen-induced aberrations in high-resolution microscopy and have multiple applications in tissue light scattering.
- Subjects
CELLS; ORGANS (Anatomy); PRESERVATION of organs, tissues, etc.; MICROSCOPY; LIGHT scattering; REFRACTIVE index
- Publication
Nature Methods, 2007, Vol 4, Issue 9, p717
- ISSN
1548-7091
- Publication type
Article
- DOI
10.1038/nmeth1078