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- Title
Point Mutations of the Sodium Channel Gene Associated with the Fenpropathrin Resistance in Scirtothrips dorsalis and Discrimination of Resistant Individuals using the PCR-RFLP Assay.
- Authors
Tomoya YOKOYAMA; Tetsuro KASHIMA; Satoshi TODA
- Abstract
We analyzed part of the sodium channel gene to clarify the molecular mechanism involved in the pyrethroid resistance of the yellow tea thrips (Scirtothrips dorsalis), and found two amino acid mutations (T929I and L1014F) known as pyrethroid-resistant factors in several other insects. The L1014F mutation was shared with all the tested thrips from a field colony. On the other hand, the T929I mutation was found only in individuals which survived in pyrethroid treatment of agriculturally recommended concentration. This might suggest that pyrethroid resistance of S. dorsalis in the field is conferred by both mutations. We developed the PCR-RFLP assay using MboI to distinguish pyrethroidresistant individuals.
- Publication
Japanese Journal of Applied Entomology & Zoology, 2014, Vol 58, Issue 1, p59
- ISSN
0021-4914
- Publication type
Article
- DOI
10.1303/jjaez.2014.59