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- Title
Honey as a Supplement to Fetal Bovine Serum in Cell Culture - A Pilot Study.
- Authors
Kannan, T. P.; Azlina, A.; Abdullah, Siti Fadilah; Abdulaziz, Q. A. M.
- Abstract
OBJECTIVE The aim of this study is to assess the efficacy of honey (Tualang) when used as a supplement to fetal bovine serum (FBS) in cell culture and to determine its effect on the expression of tumour suppressor gene. METHODS The human osteoblast cell line CRL 1543 (ATCC) was used in this study. Honey was used at different concentrations as a supplement to fetal bovine serum in the cell cultures. The cytotoxicity was assessed by two different techniques; the MTT assay and the chromosomal assay. For the MTT assay, the absorbance values were analyzed using an Elisa reader at 570 nm and for the chromosomal assay, the cytotoxicity was assessed by calculating the mitotic index values. The gene expression was evaluated using RT- PCR techniques at different cell cultures harvest timings. RESULTS In the MTT assay, the percentage of cell viability was best observed at the concentrations of 0.0195% Honey + 10% FBS (105.3%), 0.0012% Honey + 2% FBS (78.4%) and 0.0049% Honey + 5% FBS (71.4%). Mean mitotic index values 1.75 ± 0.05, 2.05 ± 0.05 and 2.3 ± 0.10 per cent were observed for the 3 different concentrations 0.0195% Honey + 10% FBS, 0.0049% Honey +5% FBS and 0.0012% Honey + 2% FBS respectively. CONCLUSIONS The MTT assay shows that FBS cannot be completely replaced by honey in cell culture. The chromosomal analyses indicate that there is enhanced cell proliferation corresponding to the decrease in the concentrations of honey and FBS.
- Subjects
HONEY; SERUM; CELL culture; GENE expression; TUMOR suppressor genes; CELL proliferation
- Publication
Malaysian Journal of Medical Sciences, 2007, Vol 14, Issue 1, p109
- ISSN
1394-195X
- Publication type
Article