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- Title
Structure of EF-G-ribosome complex in a pretranslocation state.
- Authors
Chen, Yun; Feng, Shu; Kumar, Veerendra; Ero, Rya; Gao, Yong-Gui
- Abstract
In protein synthesis, elongation factor G (EF-G) facilitates movement of tRNA-mRNA by one codon, which is coupled to the ratchet-like rotation of the ribosome complex and is triggered by EF-G-mediated GTP hydrolysis. Here we report the structure of a pretranslocational ribosome bound to Thermus thermophilus EF-G trapped with a GTP analog. The positioning of the catalytic His87 into the active site coupled to hydrophobic-gate opening involves the 23S rRNA sarcin-ricin loop and domain III of EF-G and provides a structural basis for the GTPase activation of EF-G. Interactions of the hybrid peptidyl-site-exit-site tRNA with ribosomal elements, including the entire L1 stalk and proteins S13 and S19, shed light on how formation and stabilization of the hybrid tRNA is coupled to head swiveling and body rotation of the 30S as well as to closure of the L1 stalk.
- Subjects
PROTEIN synthesis; ELONGATION factors (Biochemistry); GENETIC code; THERMUS thermophilus; GUANOSINE triphosphatase; RIBOSOME recycling factor
- Publication
Nature Structural & Molecular Biology, 2013, Vol 20, Issue 9, p1077
- ISSN
1545-9993
- Publication type
Article
- DOI
10.1038/nsmb.2645