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- Title
Analyses of growth kinetics and the development of simple media for the growth of Xanthomonas albilineans.
- Authors
Iyer, R.; Pillay, B.; Pillay, D.
- Abstract
Despite its commercial importance, little is known about the growth and nutritional requirements of Xanthomonas albilineans. To date, a chemically defined minimal medium for in vitro experimentation with X. albilineans has not been developed. We have undertaken an investigation into the growth kinetics and nutritional requirements of a strain of X. albilineans, with a view to determining whether current phenomenological models can be used to predict the growth of X. albilineans, and developing a chemically defined medium for metabolic studies. Of three phenomenological models tested, the cardinal temperature and pH model was found to be the most appropriate and precise. Growth occurred between pH 4.25 and 8.75 and was optimal at pH 6.75. Temperatures between 12°C and 34 #x00B0;C supported growth, which was optimal at 30°C. X. albilineans had an obligate requirement for organic nitrogen; however, this need not be methionine or glutamic acid. Each of 17 amino acids tested supported growth under optimized conditions. Two simple defined media that permit chemical manipulation are presented. The first is a minimal medium allowing maximum manipulation of the composition to promote growth. The second is a simple medium that promotes higher growth rates than the first while still allowing manipulation of its composition.
- Subjects
XANTHOMONAS albilineans; GRAM-negative bacteria; BACTERIAL growth; MICROBIAL growth
- Publication
South African Journal of Science, 2005, Vol 101, Issue 3/4, p197
- ISSN
0038-2353
- Publication type
Article