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- Title
Structural Basis for the KlenTaq DNA Polymerase Catalysed Incorporation of Alkene- versus Alkyne-Modified Nucleotides.
- Authors
Hottin, Audrey; Betz, Karin; Diederichs, Kay; Marx, Andreas
- Abstract
Efficient incorporation of modified nucleotides by DNA polymerases is essential for many cutting-edge biomolecular technologies. The present study compares the acceptance of either alkene- or alkyne-modified nucleotides by KlenTaq DNA polymerase and provides structural insights into how 7-deaza-adenosine and deoxyuridine with attached alkene-modifications are incorporated into the growing DNA strand. Thereby, we identified modified nucleotides that prove to be superior substrates for KlenTaq DNA polymerase compared with their natural analogues. The knowledge can be used to guide future design of functionalized nucleotide building blocks.
- Subjects
DNA polymerases; ALKENES; NUCLEOTIDES; CHEMICAL structure; BIOMOLECULE analysis; ADENOSINES
- Publication
Chemistry - A European Journal, 2017, Vol 23, Issue 9, p2109
- ISSN
0947-6539
- Publication type
Article
- DOI
10.1002/chem.201604515