We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
<sup>19</sup>F NMR Spectroscopy as a Probe of Cytoplasmic Viscosity and Weak Protein Interactions in Living Cells.
- Authors
Ye, Yansheng; Liu, Xiaoli; Zhang, Zeting; Wu, Qiong; Jiang, Bin; Jiang, Ling; Zhang, Xu; Liu, Maili; Pielak, Gary J.; Li, Conggang
- Abstract
Protein mobility in living cells is vital for cell function. Both cytosolic viscosity and weak protein-protein interactions affect mobility, but examining viscosity and weak interaction effects is challenging. Herein, we demonstrate the use of 19F NMR spectroscopy to measure cytoplasmic viscosity and to characterize nonspecific protein-protein interactions in living Escherichia coli cells. The origins of resonance broadening in Escherichia coli cells were also investigated. We found that sample inhomogeneity has a negligible effect on resonance broadening, the cytoplasmic viscosity is only about 2-3 times that of water, and ubiquitous transient weak protein-protein interactions in the cytosol play a significant role in governing the detection of proteins by using in-cell NMR spectroscopy.
- Subjects
NUCLEAR magnetic resonance spectroscopy; PROTEIN-protein interactions; CELL physiology; ESCHERICHIA coli DNA; RESONANCE broadening; CYTOSOL
- Publication
Chemistry - A European Journal, 2013, Vol 19, Issue 38, p12705
- ISSN
0947-6539
- Publication type
Article
- DOI
10.1002/chem.201301657