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- Title
Oral intake of zirconia nanoparticle alters neuronal development and behaviour of Drosophila melanogaster.
- Authors
Mishra, Monalisa; Sabat, Debabrat; Ekka, Basanti; Sahu, Swetapadma; P, Unnikannan; Dash, Priyabrat
- Abstract
Zirconia nanoparticles (ZrO NPs) have been extensively used in teeth and bone implants and thus get a chance to interact with the physiological system. The current study investigated the oral administration of various concentrations of ZrO NPs synthesized by the hydrothermal method (0.25 to 5.0 mg L) on Drosophila physiology and behaviour. The size of the currently studied nanoparticle varies from 10 to 12 nm. ZrO NPs accumulated within the gut in a concentration-dependent manner and generate reactive oxygen species (ROS) only at 2.5 and 5.0 mg L concentrations. ROS was detected by nitroblue tetrazolium (NBT) assay and (H2DCF) staining. The ROS toxicity alters the larval gut structure as revealed by DAPI staining. The NP stress of larvae affects the Drosophila development by distressing pupa count and varying the phenotypic changes in sensory organs (eye, thorax bristle, wings). Besides phenotypic changes, flawed climbing behaviour against gravity was seen in ZrO NP-treated flies. All together, for the first time, we have reported that a ROS-mediated ZrO NP toxicity alters neuronal development and functioning using Drosophila as a model organism.
- Subjects
ZIRCONIUM oxide; DROSOPHILA physiology; NITROBLUE tetrazolium; NANOMEDICINE; NEUROTECHNOLOGY (Bioengineering)
- Publication
Journal of Nanoparticle Research, 2017, Vol 19, Issue 8, p1
- ISSN
1388-0764
- Publication type
Article
- DOI
10.1007/s11051-017-3971-y