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- Title
Fine genetic mapping of a locus controlling short internode length in melon ( Cucumis melo L.).
- Authors
Hwang, Jihyun; Oh, Juyoul; Kim, Zoohyun; Staub, Jack; Chung, Sang-Min; Park, Younghoon
- Abstract
Compact and dwarfing vining habits in melon ( Cucumis melo L.; 2 n = 2 x = 24) may have commercial importance since they can contribute to the promotion of concentrated fruit set and can be planted in higher plant densities than standard vining types. A study was designed to determine the genetics of dwarfism associated with a diminutive (short internodes) melon mutant line PNU-D1 ( C. melo ssp. cantalupensis). PNU-D1 was crossed with inbred wild-type melon line PNU-WT1 ( C. melo ssp. agrestis), and resultant F progeny were then self-pollinated to produce an F population that segregated as dwarf and vining plant types. Primary stem length of F progeny assessed under greenhouse conditions indicated that a single recessive gene, designated mdw1, controlled dwarfism in this population. To identify the chromosomal location associated with mdw1, an simple sequence repeat (SSR)-based genetic linkage map was constructed using 94 F progeny. Using 76 SSR markers positioned on 15 linkage groups spanning 462.84 cM, the location of mdw1 was localized to Chromosome 7. Using the putative dwarfing-associated genes, fine genetic mapping of the mdw1 genomic region was facilitated with 1,194 F progeny that defined the genetic distance between mdw1 and cytokinin oxidase gene, a candidate gene for compact growth habit ( cp) in cucumber, to be 1.7 cM. The candidate gene ERECTA (serin/threonine kinase) and UBI (ubiquitin) were also mapped to genomic regions flanking mdw1 at distances of 0.6 and 1.2 cM, respectively.
- Subjects
PLANT gene mapping; MELONS; LOCUS in plant genetics; DWARFISM; PLANT chromosomes; PLANT mutation; PLANTS
- Publication
Molecular Breeding, 2014, Vol 34, Issue 3, p949
- ISSN
1380-3743
- Publication type
Article
- DOI
10.1007/s11032-014-0088-1